Dynamic Bed Reactor technology in CellBRx® Bioreactors:
The Universal Solution for most efficient and high cell density perfusion cell culture with ultimate Simplicity and Scalability.
The existing cell culture technologies for upstream processing includes devices and bioreactor systems with increased surface area using multiple plates, parallel staked discs, cell carriers in packed bed, rolled membranes and microcarriers. The aforesaid technologies suffer from common limitations i.e. scalability, efficiency and affordability.
The core objective is to develop an innovative bioreactor system with DBR technology to address the existing constraints and limitations to produce vaccines, recombinant therapeutics and stem cells.
Surface area for cell attachment and growth has been increased drastically with ensured homogenous mixing of media and nutrients along with provision of efficient harvesting of cells using automation. The innovative DBR technology design offers a homogeneous mixing and maximum surface area for adherent cell cultures. Also, provide ease of operation, affordability and efficiency.
The proprietary DBR technology employs parallel discs stacked on multiple shafts i.e. central and peripheral shafts. The intermingling arrangement of the discs loaded on central and peripheral shafts ensures efficient mixing and absence of non-homogeneous conditions throughout the culture support matrix.
Scalability
CellBRx® bioreactors runs on truly scalable technology to provide linear scalability from R&D to manufacturing scale.
Efficiency
They are the first in class bioreactors for most efficient high cell density adherent cell culture.
Affordability
Small facility footprints with very high volumetric productivity makes the CellBRx® bioreactors most affordable and preferable tools for biologics production
Simplicity
Integrated perfusion logic and robust automation software turns these bioreactors, the most simplest yet fully automated culture platforms.
Single-Use & customizable
Pre-calibrated, Pre-validated and fully customizable manufacturing from 0.25L to 500L Bioreactors
Comparative analysis of DBR technology to other marketed products:
| Performance criteria | Cell factories | Packed bed bioreactors | Microcarriers (STRs) | Roller Bottles | CellBRx® SUBs (200L) |
|---|---|---|---|---|---|
| Scalability | + | + | ++ | ++ | +++++ |
| Surface Area (SA) / Unit | 0.64 m2 | 0.085 m2 | 264 m2 | 500 m2 | 1500 m2 |
| SA / volume ratio | 3.2 cm2/ml | 5.4 cm2/ml | 13.2 cm2/ml | 71.2 cm2/ml | 75 cm2/ml |
| Mixing | NA | +++ | +++ | + | +++++ |
| Ease of handling | + | + | ++ | ++ | ++++ |
| Contamination risk | ++++ | ++++ | +++ | ++ | + |
| Cell Harvest feasibility | ++++ | ++++ | ++ | + | ++++ |
| Footprints | ++++ | +++++ | +++ | +++ | + |
| Automation | No | No | Yes | Yes | Yes |
| Equivalent no’s of units to CellBRx® | 2344 | 17647 | 5.7 | 3 | 1 |
| Cost of eq. units | 12 X | 8 X | 7 X | 5 X | 1 X |
The existing constraints of the bioprocessing industry will be addressed in single-use bioreactors by using Dynamic Bed Reactor technology, which will offer following benefits.
- Deliver fully scalable culture systems from lab to production scale with improved mixing and efficiencies for cell culture applications
- Decrease contamination risk with single-use, closed systems
- Reduce upstream validation requirements hence increase speed of medicine availability in market
- Improve cell health and quality, resulting in homogeneous protein expression
- Reduce costs in the form of capital expenditures, labour, facilities and materials, while increasing protein/cell yield per square foot of production space
CellBRx® bioreactors are the only systems of its kind employing Dynamic Bed Reactor Technology in the bioprocessing industry and is the most versatile, functional, and efficient system in today’s market. The CellBRx® Bioreactors are available in several configurations. Bioreactors range can be fully customized to fulfill the client requirements and will be readily available in various capacity & sizes, ranging from 1 L to 200 L capacity.
For process development and Research work, CellBRx® micro 1L are the world’s first perfusion integrated microbioreactors. Pilot scale bioreactors CellBRx® 5L are for small scale manufacturing and pilot scale production. For large scale production and manufacturing of biologics, CellBRx® 50L to 200L are available with capacities ranging from 50L to 200L working volume.
| Configurations of CellBRx® Bioreactors form R&D to manufacturing scale | ||||||
|---|---|---|---|---|---|---|
| Scale Variants | ||||||
| Single-use Bioreactors | BRx Volume (Liters) | Surface Area in single-use bioreactor vessel (m2) | Equivalent Roller Bottles (850cm2) | Equivalent Cell Stacks or Cell Factories (CF10) | ||
| CellBRx® 1L | 1 | 1m2 | 11.8 | 1.6 | ||
| CellBRx® 5L | 5 | 10m2 | 118 | 16 | ||
| CellBRx® 50L | 50 | 250 m2 | 2941 | 391 | ||
| CellBRx® 200L | 200 | 1500 m2 | 17647 | 2344 | ||
For Stem cell culture applications, i.e. cell therapies and gene therapies, the CellBRx® bioreactor support matrix surfaces can be customized to support growth and differentiation of different types of stem cells. Customization is also available to maximize the cell harvesting efficiency of the bioreactor system.
To arrange for live demonstration of the CellBRx® Bioreactors at your facility, click here.