PerfBRx® Advanced Culture Technologies
In the realm of suspension cell culture, traditional fed-batch bioreactor systems, which usually run between 7–21 days, have long been the standard. These systems involve cells consuming media nutrients and releasing both toxic metabolites and the targeted protein into the media. At the end of the run, the protein is then separated from the cell mass. Even with numerous technological enhancements, fed-batch processes still grapple with issues such as large manufacturing footprints and scalability challenges.
In contrast, perfusion bioreactors, a pivotal element in cutting-edge culture technologies, offer extended cell culture periods, even spanning several months. This is achieved by continuously feeding the cells with fresh media and concurrently removing the spent media.
When it comes to separating cells from the culture fluid during spent media removal, several methods are employed. The tangential flow filtration system (TFF) and its counterpart, the alternating tangential filtration system (ATF), are predominant. They maintain cells within the bioreactor while facilitating media removal. Centrifugation offers an alternative approach, isolating cells and subsequently reintroducing them into the bioreactor.
However, there exist limitations with current perfusion system bioreactor separation devices. Issues such as limited perfusion batch scalability (maxing out at 1000L), frequent filter blockages, and the exertion of shearing forces on cells during separation present significant challenges.
As a result, the bioprocess industry still awaits the development of both perfusion-enabled micro bioreactors (100 ml) and upscaled versions of perfusion bioreactors exceeding 2000L.
Enter PerfBRx®. Representing the pinnacle of modern culture technologies, these bioreactors are a groundbreaking fusion of high-cell density perfusion cell culture and state-of-the-art design. They proudly hold the distinction of being the world’s premier perfusion-integrated single-use bioreactors. With an unparalleled novel separation device, they exhibit linear scalability ranging from a mere 0.1L to an impressive 5000L.
Modern advancements in biochemistry have ushered in a new era of cell culture techniques. The integration of the tangential flow filtration system has revolutionized the efficiency of perfusion bioreactors. This technology enhances the efficacy of perfusion system bioreactor processes, establishing it as a cornerstone in state-of-the-art culture technologies. The promise it holds for scalability and reduced system clogs makes it a sought-after tool for professionals aiming for cutting-edge results.
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Technology is under development.